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The Bartholin's glands, located near the vaginal opening, were originally considered the primary source for vaginal lubrication, but further examination showed that they provide only a few drops of mucus. The significant majority of vaginal lubrication is now believed to be provided by plasma seepage from the vaginal walls, which is called vaginal transudation.

Vaginal transudation, which initially forms as sweat-like droplets, is caused by vascular engorgement of the vagina (vasocongestion), resulting in the pressure inside the capillaries increasing the transudation of plasma through the vaginal epithelium.